5.8s rrna sequence and secondary structure in parabronema skrjabini and related habronematidae species

نویسندگان

seyed sajjad hasheminasab

چکیده

background: genomic dna was isolated from parabronema skrjabini . rrna region was amplified and sequenced. methods : the rna secondary structure was predicted using mfold software (http://mfold.rit.albany.edu). the secondary structure with bulge, hairpins, helices, interior, external and multi loops was predicted for 5.8srdna of our sequence of p. skrjabini and a sequence of p. skrjabini and two species of habronema ( h. microstoma and h. muscae ) in genbank. rna motifs were predicted by meme program version 4.10.2.  results : the length of 5.8s rrna sequence for p. skrjabini#1 , p. skrjabini#2 , h. microstoma and h. muscae was 158, 156, 127 and 127bp, and the dg required for the formation of the secondary structure was -70.50, -56.40, -41.50 and -41.40 kcal/mol, respectively. common structural elements were initially recognized with the help of mfold by screening for thermodynamically optimal and suboptimal secondary structures (default settings, with t = 37 °c). the energy levels of the presumptive secondary structures were then calculated with mfold at the dna level. both motifs and the sequence of p. skrjabini#1 were completely different from the other analyzed samples. this difference might be due to the differences in host and geographical area. conclusion : this is the first molecular study of p. skrjabini in sheep, which could be further used in the structure modeling across habronematidae.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

5.8S rRNA Sequence and Secondary Structure in Parabronema skrjabini and Related Habronematidae Species

BACKGROUND Genomic DNA was isolated from Parabronema skrjabini. rRNA region was amplified and sequenced. METHODS The RNA secondary structure was predicted using mfold software ( http://mfold.rit.albany.edu ). The secondary structure with bulge, hairpins, helices, interior, external and multi loops was predicted for 5.8srDNA of our sequence of P. skrjabini and a sequence of P. skrjabini and tw...

متن کامل

Molecular characterization of the first internal transcribed spacer of rDNA of Parabronema skrjabini for the first time in sheep.

Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular aspects of Parabronema skrjabini in sheep. The worms were collected from sheep in Sanandaj (west of Iran). The first internal transcribed spacer (ITS) of ribosomal DNA (rDNA) nucleotide fragments of ...

متن کامل

Molecular and morphological characterization of Parabronema skrjabini of sheep and goats at three different geographical zones in Iran.

Parabronema skrjabini is a spirurid nematode of the family Habronematidae that lives in the abomasum of ruminants such as sheep and goats. The purpose of this study was to investigate the molecular and morphological aspects of Parabronema skrjabini in sheep and goats in Iran. The worms were collected from these animal species from three different regions. An internal transcribed spacer 2 riboso...

متن کامل

Sequence and secondary structure of 5.8S rRNA in the tick, Ixodes scapularis.

While sequence homology among eukaryotic 5.8S rRNAs is relatively high, even among distantly related organisms (1), the secondary structure of this region, although similarly conserved, does not rely completely on sequence integrity. That is, the functional folding pattern remains the same while utilizing different rRNA base pairs achieved through compensatory mutation. Ticks in the genus Ixode...

متن کامل

manipulation in dubbing and subtitling

پژوهش حاضر در چارچوب مکتب دستکاری قرار گرفت و با استفاده از تقسیم بندی دوکات (2007) از شیوه های دستکاری، به دنبال یافتن پاسخ برای پرسش های زیر بود: 1-رایج ترین شیوه دستکاری در دوبله فیلم ها کدام است؟ 2-رایج ترین شیوه دستکاری در زیرنویس فیلم ها کدام است؟ 3-دستکاری در دوبله فیلم ها رایج تر است یا در زیرنویس آن ها؟ این پژوهش از نوع تحقیقات توصیفی- مقایسه ای و پیکره ای می باشد. پیکره تحقیق شا...

The primary and secondary structure of yeast 26S rRNA.

We present the sequence of the 26S rRNA of the yeast Saccharomyces carlsbergensis as inferred from the gene sequence. The molecule is 3393 nucleotides long and consists of 48% G+C; 30 of the 43 methyl groups can be located in the sequence. Starting from the recently proposed structure of E. coli 23S rRNA (see ref. 25) we constructed a secondary structure model for yeast 26S rRNA. This structure...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of parasitology

جلد ۱۱، شماره ۲، صفحات ۲۵۳-۲۵۸

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023